New dudes have been attending feel the spunk examination was coached at the time of fulfilling to keep sexual abstinence having a time period of less than six months before the date out-of range 11 . This new seminal sample is gotten by genital stimulation during the a great sterile vial previously identified towards patient password. After range, the vial was put hvordan er postordrebrude lovlige by patient himself so you can a lab professional where he placed they on incubator on 37 °C for about 60 minutes to possess take to liquefaction. At the end of liquefaction, the latest test was transferred to a graduated conical tube that have a sterile Pasteur pipette to own frequency verification. From inside the whole period of studies, this new samples were drawn in an identical laboratory and by the newest same people regarding biologists with experience with seminal assesses.

Evaluation out-of jizz concentration

Seminal sample dilution was performed in formalin (50 g NAHCO₃, 10 mL 35% formalin, and distilled water to 1 liter), following the ratio determined by the biologist responsible for the examination. After dilution, an aliquot was placed on either side of the Neubauer chamber. After a period for cell sedimentation, the chamber was analyzed by optical microscope with 10X to 40X magnification, counting the amount of sperm present in the four squares corresponding to the edges, as well as in the central square. (more…)